ABSTRACT
Objective To investigate the health status of the medical staffs in our hospital , find risk factors and make corresponding strategies to improve health of status .Methods Results of physical examination on 1203 medical staffs were analyzed by SAS 9.2.Results Total residual test ratio is 9.3%, and the residual test ratio of male was significantly higher than female (χ2=38.399,p <.0001).The prevalence ratio of all subject is 60.9%, and there was no significant difference between male and female , but the higher age category, the higher prevalence ratio (χ2=211.4037,p <.0001).The top 5 diseases our staffs suffering are waist and cervical vertebra disease , thyroid nodule, hyperlipidemia, breast hyperplasia and fatty liver, in which the prevalence ratio of thyroid nodule and hyperlipidemia was different between male and female .Conclusion The health status of our medical staffs should be improved by paying high attention to medical examination and health management .
ABSTRACT
Objective To study the different expressions of neurotensin (NT) at gene and protein level in orthotopic model of prostate cancer . Methods The animal models of androgen dependent prostate cancer(ADPC,castrated for 3 days) and androgen independent prostate cancer(AIPC) were established by planting tumor tissue or undergoing surgical castra-tion. Affymetrix microarray technology was carried out to compare the gene expressions of NT. The result was verified by qRT-PCR. HE staining was used to observe the change of pathology. ELSIA and immunohistochemistry technology were fi-nally performed to detect protein expression of prostate-specific antigen(PSA) and NT in three different groups of prostate cancer tumor tissues. Results The expression of NT was 5.10 times higher in AIPC group than that in ADPC group. The ex-pression of NT was 0.33 times lower in castrated 3-day group than that in ADPC group. Results of RT-PCR and qRT-PCR showed that the expression levels of NT gene were 1.41 and 7.27 times respectively higher in AIPC group than that in ADPC group,but the expression levels of NT gene were 0.78 and 0.46 times respectively lower in castrated 3-day group than that in ADPC group (P<0.05). HE results showed that nuclear atypia and tumor structure in three groups. Immunohistochemistry and ELISA results showed that the values of PSA and NT were (0.48±0.03) and (0.031±0.008)μg/L in ADPC group;(0.17± 0.03) and (0.021±0.004)μg/L in castrated 3-day group,and (0.87±0.02) and (0.042±0.010)μg/L in AIPC group. There were significantly lower expressions of NT and PSA in castrated 3-day group that those of ADPC group (P<0.01). Conclusion In the transition from ADPC to AIPC, the over-expression of NT suggested that NT may be associated with prostate cancer progression. NT may be used as a new therapeutic target and specific diagnostic method of AIPC.